Fig. 3

DTL overexpression reduced PDCD4 protein level. a DTL cDNA was constructed into PLVX-AcGFP-N1 vector and transfected into 293 T cells. PDCD4 protein level was detected using Western blotting. b Three different DTL interference sequences with a scramble sequence were constructed in PLKO.1-puro vector and transfected into 293 T cells. PDCD4 protein level was detected. c DTL stable overexpression cell line was established in H1650 and the PDCD4 protein level was detected. d 10 mmol/L MG132 was added for 6 h into 293 T cells with DTL overexpression. The PDCD4 protein level was detected by Western blot. e Breast cancer tissues were collected and stained by IHC using DTL and PDCD4 antibodies. The scale bar represents 50 μm. f Quantitative analysis were performed by Image-pro Plus software and correlation analysis was performed by EXCEL. *, P < 0.05, **, P < 0.01, NS, no significance based on the Student t test. All results are from three or four independent experiments. Error bars, SD