Fig. 5

DTL overexpression in cancer tissues and cells was related to patient prognosis. a GEO database analysis of DTL expression in breast and lung cancers. b Proteins of eight pairs of lung and breast cancer tissues were extracted. Western blot analysis showed DTL expression levels in cancer and adjacent tissues. NSCLC means non-small cell lung cancer and BRCA means breast cancer. c DTL mRNA levels of 37 pairs of lung cancer and 24 pairs of breast cancer with their adjacent normal tissues were detected by real-time quantitative PCR. d IHC staining of human breast tissues and breast cancer tissues for DTL to show their expression differences. Statistic results were shown in right diagram using IOD analysis in Image Pro Plus software. The scale bar represents 50 μm. e Western blot to assess the DTL protein expression in lung and breast cancer cell lines. f Survival curves of breast and lung cancer patients with alteration DTL levels were calculated in Kaplan-Meier method using this KM plotter. The patient numbers were shown below each diagram. *, P < 0.05, **, P < 0.01 based on the Student t test. All results are from three or four independent experiments. Error bars, SD