Fig. 7

PDCD4 mediated DTL functions in cancer cells. a Three different PDCD4 interference sequences and a scramble sequence were constructed in PLKO.1-puro vector and packaged into lentivirus to infect MDA-MB-231 cells. Knockdown efficiency was detected by Western blot. b MTT assay results showed that knockdown of PDCD4 rescued the proliferation ability insufficient in DTL silencing cells. c Colony formation assay was performed in MDA-MB-231 cells. d Statistical analysis results of colony formation were as shown. e Representative transwell and matrigel assays performed in MDA-MB-231 cells were shown. f Statistical analysis of transwell and matrigel assays. g Wound healing assay was performed in the MDA-MB-231 cells with silencing both DTL and PDCD4. Uncovered areas in the wound healing assay were quantified as a percentage of the original wound area. h Statistical analysis of wound healing assays. The scale bars represent 50 μm. *, P < 0.05, **, P < 0.01, NS, no significance based on the Student t test. All results are from three or four independent experiments. Error bars, SD