Fig. 5

ERβ induces autophagy via CLDN6-mediated increase in beclin1. Expression of autophagy-related proteins was detected by using western blot in DPN-treated cells and in CLDN6-knockdown and beclin1-knockdown DPN-treated MDA-MB-231 (a) and SK-BR-3/ERβ (b) cells. Actin served as a loading control. c Expression of autophagy-related proteins was detected by using western blot in CLDN6-overexpressing MDA-MB-231, SK-BR-3 and MCF-7 cells and CLDN6-overexpressing beclin1-knockdown cells. d Western blot analysis of ZO-1 and UVRAG in DPN-treated and CLDN6-overexpressing MDA-MB-231 cells. e-f Co-IP assays were performed to detect the interaction between beclin1 and CLDN6 in MDA-MB-231 cells treated with DPN. Data are presented as mean ± SD. The data shown are representative results of three independent experiments. *P < 0.05