Fig. 7

Eugenol/cisplatin combination strongly suppresses OCSC self-renewal and ameliorates disease-free survival of animals. a Experimental design: Freshly dissociated live-banked cells from tissues harvested from control, eugenol-, cisplatin- and combination-treated mice as described in Fig. 6 were either left unsorted or were sorted before further analysis as shown. b Dissociated cells were cryopreserved, and then were cultured for 3 weeks in a semi-solid agarose medium for colony formation analysis. c Dissociated cells from freshly harvested xenograft tumors were FACS sorted for CD44 and ALDH. d and e. Freshly dissociated cells from xenograft tumors were re-implanted into Nu/J mice (n = 5) for 8 weeks as described in Fig. 6a and the numbers of palpable tumors are shown in Table (d), and e Representatives of harvested xenograft tumors from control and treatment groups. f and g. Tumor masses [at harvest, ‘p’ values from Eug vs Eug + Cis = **p 0.01; and Cis vs Eug + Cis = ***p 0.001, respectively by unpaired two-sided t-Test]. h and i. Tumor-free survival analysis (Log-rank) of OV2774 (p = 0.0001) and SKOV3 (p = 0.002) xenografts