Fig. 2

20-HETE or PGE₂ partly reverses the antiangiogenic activities of the screened molecules in the zebrafish embryo. a The zebrafish embryos in 96-well microplate were treated with the indicated concentrations of compounds [isoliquiritigenin (ISL), glabridin (GLA) and hesperetin (HSP), 10 μM] alone, the compounds (10 μM) plus 20-HETE or PGE₂, and vehicle for 48 h. Selected images of angiogenesis in the zebrafish embryo in different groups as shown in (a); Average vessel length for each group (b). The values are presented as the mean ± SEM, n = 20. ^*P < 0.05; ^**P < 0.01 vs. control; ^#P < 0.05; ^##P < 0.01 vs. ISL, GLA or HSP-treated groups