Fig. 3

20-HETE or PGE₂ partly reverses the antiangiogenic activities of isoliquiritigenin (ISL) in the rabbit cornea. A 7–0 black silk was used to pass the rabbit corneal stroma 1 mm from the horizontal to the limbus with a length of 3 mm to induce corneal neovascularization model. One week after suturing, the suture was removed, and the flavonoid ISL (1 and 2 mg/ml), ISL (2 mg/ml) plus 20-HETE or PGE₂, and vehicle were performed twice a day for a week in eye drops. Photographs were taken with a stereomicroscope at day 0, 3 and 7 after treatment (a), and the vessel areas were quantified using image pro plus 6.0 (b). The values are presented as the mean ± SEM, n = 6. ^*P < 0.05; ^**P < 0.01 vs. control; ^#P < 0.05; ^##P < 0.01 vs. ISL or GLA-treated groups