Fig. 1

Relative AFAP1-AS1 expression in OS tissues and cell lines. The effects of AFAP1-AS1 on OS cell proliferation in vitro. a Expression of AFAP1-AS1 in OS tissue (n = 8) was significantly higher compared with adjacent non-cancerous tissues (n = 8), P = 0.0078. b Expression of AFAP1-AS1 in OS cell lines (MNNG/HOS, MG63, SaOS-2 and U2OS) was significantly higher compared with normal human osteoblasts cell line (hFOB 1.19). c Transfection with siRNA (si-AFAP1-AS1 1# and 2#) resulted in significantly lower AFAP1-AS1 expression. d After transfection, in the groups of si-AFAP1-AS1 1# and 2#, the OS cells (MNNG/HOS and U2OS) exhibited significantly decreased growth activity compared with the control. e Knockdown of AFAP1-AS1 significantly inhibited colony formation of MNNG/HOS and U2OS cells. f AFAP1-AS1 expression in nucleus was higher than in cytosol in OS cells (MNNG/HOS and U2OS). *P < 0.05, **P < 0.01