Fig. 3
Effect of AFAP1-AS1 knockdown on osteosarcoma in vivo. a, b and c The volume and weight of tumor xenograft in nude mice with AFAP1-AS1 knockdown was reduced compared to the scrambled nude mice. Tumor xenograft growth in the AFAP1-AS1 knockdown nude mice was slower than that in the control group. d The average expression of AFAP1-AS1 was decreased in nude mice with AFAP1-AS1 knockdown compared to the scrambled nude mice. e, h and i The tumor tissues were under HE and IHC staining using antibodies against Ki-67 and vimentin. f and g IHC analysis demonstrated a significant decrease in average Ki67 and vimentin expression in the AFAP1-AS1 knockdown group compared to that of the scrambled group. *P < 0.05, **P < 0.01, ***P < 0.001