Fig. 1

LINC00460 facilitated HNSCC cell proliferation, migration and invasion in vitro. a The relative expression of LINC00460 in 7 HNSCC cell lines and normal oral epithelial cells (Normal cell) was detected by qRT-PCR. b The relative expression of LINC00460 in CAL-27 and HN30 cells transfected with SS-LINC00460 was detected at 24 and 48 h by qRT-PCR. c The relative expression of LINC00460 in CAL-27 and SCC-9 cells stably transduced with LINC00460 vector (LINC00460) was detected by qRT-PCR. d, e The effect of LINC00460 on cell proliferation was evaluated with CAL-27 and HN30 cells transfected with SS-LINC00460 (d) and with CAL-27 and SCC-9 cells stably transduced with LINC00460 (e) by CCK-8 assays. f, g The colonizing ability of CAL-27 and HN30 cells transfected with SS-LINC00460 (f) and of CAL-27 and SCC-9 cells stably transduced with LINC00460 (g) was determined by colony formation assays. h, i The cell migration and invasion abilities of CAL-27 and HN30 cells transfected with SS-LINC00460 (h) and of CAL-27 and SCC-9 cells stably transduced with LINC00460 (i) were determined by transwell assays, Scale bar: 1000 μm. (NC: negative control; SS-LINC00460: LINC00460 Smart Silencer; LINC00460 vector: LINC00460 lentiviral expression vector.) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001