Fig. 4

PRDX1 physically interacted with LINC00460 and affected HNSCC cell proliferation and migration. a Western blot analysis of PRDX1 following RNA pull-down assays with LINC00460 chromatin isolation by LINC00460 probes in CAL-27 cells stably transduced with LINC00460. b, c Relative expression of PRDX1 in CAL-27 cells infected with the PRDX1 lentiviral expression vector with HA-Tag (PRDX1-HA) as detected by Western blot analysis (b) and qRT-PCR (c). d qRT-PCR analysis of LINC00460 enriched by anti-HA in CAL-27 cells stably transduced with PRDX1-HA in RIP assays. Ten specific primers for LINC00460 were used to detect the results of RNA enrichment. e Construction of the PRDX1-Mut vector, which changed AAG to AGG from nucleotides 358 to 360, and the relative expression of PRDX1-WT and PRDX1-Mut vectors as detected by Western blot analysis in CAL-27 cells. f qRT-PCR analysis of LINC00460 enriched by anti-HA in CAL-27 cells transfected with PRDX1-HA-WT (WT) and PRDX1-HA-Mut (Mut) vectors in RIP assays. g Construction of the LINC00460-Mut vector, which changed TTGTGGC into GGAGAAT from nucleotides 320 to 326. h Western blot of PRDX1 following RNA pull-down assays retrieved by LINC00460 probes in CAL-27 cells transfected with LINC00460-WT (WT) and LINC00460-Mut (Mut) vectors. i, j The relative expression of PRDX1 in 7 HNSCC cell lines and normal oral epithelial cells (Normal cell) as determined by qRT-PCR (i) and Western blot analysis (j). k Silencing efficiency of si-PRDX1 in CAL-27 cells as detected by qRT-PCR. l The effect of PRDX1 expression on cell proliferation was evaluated with CAL-27 cells transfected with si-PRDX1 by CCK-8 assays. m The colonizing ability of CAL-27 cells transfected with si-PRDX1 was determined by colony formation assays. n The cell migration abilities of CAL-27 cells transfected with si-PRDX1 were determined by transwell assays, Scale bar: 1000 μm. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: no significance