Fig. 3

Thal increases pericyte coverage and endothelial tight-junctions. a Double staining for CD31 (Green) and α-SMA (Red) and quantification of percentage of α-SMA⁺ vessels in tumor sections from 4 T1 and CT26 models. Bars: 50 μm. b Staining for Claudin-5 (Red) indicating endothelial tight-junctions in tumor sections from 4 T1 and CT26 models. Tight-junctions are presented as a percentage of Claudin-5⁺/CD31⁺ vessels. Bars: 50 μm. c 4 T1 and CT26 tumors were stained for CD31 (Blue) and VE-cadherin (White) and quantified by percentage of tortuous vessels, and VE-cadherin⁺ vessels. Bars: 25 μm. Quantitative data are represented as mean ± SD. All p values were calculated by one-way ANOVA analysis followed by Dunnett’s multiple comparison test. *p < 0.05, ** p < 0.01, ***p < 0.001