Fig. 5
The effect of co-expression linc01296 and miR-26a on CRC progression. a Flow cytometry showed O-linked glycosylation level detected by fluorescein isothiocyanate (FITC)-conjugated VVA on the cell surface of transfected CRC cells. b CCK8 assay showed variant growth rate of transfected CRC cells. c Colony formation assay detected proliferative formation with different treated CRC cells. d Aggressiveness changed was determined by transwell assay, the migratory and invasive cells were counted. e CRC cells were treated with 5-FU, and the cell viability was detected by CCK8 assay. f IC50 values of treated CRC cells were calculated. g The effect of 5-FU on CRC cell proliferation was determined by colony formation assay. h Flow cytometry showed the apoptosis rate of transfected CRC cells in response to 5-FU. i FITC-VVA on transfected CRC cells surface was detected by flow cytometry. j The growth curves of transfected CRC cells were pictured after conducting CCK8 assay. k Proliferation of treated CRC cells was examined by colony formation assay. l Migration and invasion were observed in transfected SW620 cells. m With 5-FU treatment, the cell viability was determined by CCK8 assay. n The IC50 values were subsequently calculated. o 5-FU resistant CRC cells were transfected with linc01296 or miR-26a mimic, and the colony formation was counted in response to 5-FU. p The apoptosis rate of transfected CRC cells was detected after 5-FU treatment by flow cytometry. The data were means ± SD of three independent assays (*P < 0.05)