Fig. 2

CBP impairs mesothelioma tumorigenic properties. a) The effect of CPB on colony forming ability of MM cell lines was analysed after 7 days of culture post CPB treatment. For quantification, colonies with at least 50 cells were considered. b) Invasive capability of human MM cells was determined using transwell invasion assay with Matrigel after 24 h CBP treatment as described in “Methods”. For each cell line five random fields in each well were counted under a microscope. Bright field images magnification: × 10. Representative micrographs shown in a) and b) were obtained after crystal violet staining. Histograms report average of colony or cell numbers respectively (% of control). c) Cell migration activity of mesothelioma cell lines was examined by wound healing assay. The wound closure rate was measured by detecting the closure distance after 24 h. Representative phase contrast images at 0 and at 24 h after CBP treatment (up) and quantification of the distance (down) are shown. Magnification of the upper panels: × 10. The indicators represent the average ± SD of independent experiments (n = 3). Statistically significant difference compared to untreated cells: **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. CTRL: untreated cells