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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: S1PR1 promotes proliferation and inhibits apoptosis of esophageal squamous cell carcinoma through activating STAT3 pathway

Fig. 3

The effects of S1PR1 overexpression on cell proliferation, apoptosis of ESCC cells. a CCK-8 assay analysis of kyse150 and TE-13 cells infected with LV-NC or LV-S1PR1 respectively. b Flow cytometry analysis of cell cycle distribution proportion in kyse150 and TE-13 cells infected with LV-NC or LV-S1PR1 respectively. c Colony formation assay of kyse150 cells infected with LV-NC or LV-S1PR1 respectively treated with indicated concentrations of PBS and cDDP. d Flow cytometry analysis of apoptotic cells in kyse150 cells infected with LV-NC or LV-S1PR1 respectively treated with indicated concentrations of PBS and cDDP. e Immunofluorescent staining of c-caspase3 of kyse150 and TE-13 cells infected with LV-NC or LV-S1PR1 respectively. f Western blot analysis of S1PR1, PCNA, cyclinD1, Bcl-xL, c-caspase3 and GAPDH in kyse150 and TE-13 cells infected with LV-NC or LV-S1PR1 respectively

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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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