Fig. 5

P-STAT3 inhibition inhibited proliferation and enhanced apoptosis of ESCC cells with S1PR1 knockdown or overexpression. a Colony formation assay of kyse150 and TE-13 cells with S1PR1 knockdown or overexpression treated with/without SH-4-54. b Flow cytometry analysis of apoptotic cells in kyse150 and TE-13 cells with S1PR1 knockdown or overexpression treated with/without SH-4-54. c Western blot analysis of PCNA, p-STAT3, STAT3, Bcl-xL, c-caspase3 and GAPDH in kyse150 and TE-13 cells with S1PR1 knockdown or overexpression treated with/without SH-4-54. d Immunofluorescent staining of c-caspase3 of kyse150 and TE-13 cells with S1PR1 knockdown or overexpression treated with/without SH-4-54