Fig. 2

miR-504 suppresses the ME properties and EMT of GBM cells. a qRT-PCR detection of miR-504 expression in U87 and U373 cells transfected with miR-504 overexpression and inhibition vectors. a Representative images of GBM cell morphological changes after miR-504 transfection (scale bars = 100 μm). (c, d) Representative results of wound healing assays (c) and Transwell assays (d) showing the effect of miR-504 overexpression and inhibition on the migration and invasive abilities of GBM cells (scale bars = 100 μm). e Western blot analysis of the levels of representative epithelial and ME markers. f Immunofluorescence examination of ME marker expression in U87 and U373 cells transfected with miR-504 or miR-NC (red, vimentin; blue, DAPI; scale bars = 100 μm). Data are the mean ± SD of three independent experiments. *P < 0.05,**P < 0.01, ***P < 0.001