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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: miR-504 suppresses mesenchymal phenotype of glioblastoma by directly targeting the FZD7-mediated Wnt–β-catenin pathway

Fig. 4

miR-504 targets the FZD7 3′UTR directly. a Venn diagram identifying the overlap between predicted genes and miR-504–negatively related genes. b The predicted target site of FZD7 3′UTR binding to miR-504. c Pearson correlation analysis of miR-504 and FZD7 in TCGA. d TCGA data analysis of FZD7 expression levels between miR-504 high-expression tumors (n = 129) and miR-504 low-expression tumors (n = 388). e Measurement of firefly/Renilla luciferase activity in HEK-293 T cells co-transfected with wild-type-FZD7–3′-UTR or mutant-type-FZD7–3′-UTR reporters and NC mimic or miR-504 mimic. f, g qRT-PCR determination of FZD7 mRNA levels in U87 and U373 cells after transfection with miR-504, miR-NC, anti-NC, or anti-miR-504. h Western blot analysis of FZD7 protein levels in U87 and U373 cells after transfection with miR-504, miR-NC, anti-NC or anti-miR-504 . i Immunofluorescence examination of FZD7 expression in U87 cells transfected with miR-504 or miR-NC (red, FZD7; green, GFP; blue, DAPI; scale bars = 100 μm). j Immunohistochemistry of FZD7 in xenograft sections (scale bar = 50 μm). Data are the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001

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