Fig. 2From: Modulating lncRNA SNHG15/CDK6/miR-627 circuit by palbociclib, overcomes temozolomide resistance and reduces M2-polarization of glioma associated microglia in glioblastoma multiformeA higher level of lncSNHG15 in TMZ resistant GBM cells and associated with GBM malignancy (a) Comparative lncSNHG15 expression between TMZ resistant and sensitive clinical GBM samples. Real-time PCR analysis showed that a significantly higher level of lncSNGH15 in samples from patients who are non-responsive towards TMZ as compared to ones with responsiveness. TMZ-resistant cells showed an enhanced colony (b) and neurosphere (c) forming ability over TMZ-sensitive counterparts. d Comparative flow cytometric analysis also showed that TMZ-R cells contained a significantly higher percentages of CD133+ population (approximately 30% more) than their TMZ-S counterparts. e Comparative qPCR and western blot analysis of TMZ-resistant and TMZ-sensitive GBM cells. The lncSNHG15 was significantly higher in the TMZ-R cells as compared to TMZ-S cells. Also, the expression of stemness markers, Sox2, β-catenin and oncogenic markers EGFR and CDK6 were significantly lower in the TMZ-sensitive cells as compared to its TMZ-resistant counterpart. Neural differentiation marker GFAP was slightly higher in the TMZ-sensitive GBM cellsBack to article page