Fig. 2

Conjugated peptide antigen as a preventive metastasis cancer vaccine. (a) Location of antigen Epi-SP in whole protein. (b) The sequences of mouse and human Epi-SP. (c) The Location of Epi-SP (red) in the structure model. (d) Immunization protocol. (e) The survival curves with immunization. Mice were sacrificed on day 19 to assess metastasis. (f) Number of metastasis nodule with immunization (Left). The representative images of metastasis (Right). (g) The levels of 4 T1 metastasis inhibition with immunization of Epi-SP are similar to the levels of 4 T1EpiKD. 4 T1C: 4 T1Con, 4 T1KD: 4 T1EpiKD. (h) Antibody specificity to Epi-SP and Epi-Sc. Antigens were immunized three times and antibodies were examined by ELISA. SP: EPi-SP, Sc: EPi-Sc. (i) Antiserum from the immunized mice blocks transendothelial migration. Overall scheme of the experiment is shown on the left, cells migrated through MS1 endothelial cells toward the opposite side are shown on the right. Average and standard error of means are shown