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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Targeting metastatic breast cancer with peptide epitopes derived from autocatalytic loop of Prss14/ST14 membrane serine protease and with monoclonal antibodies

Fig. 3

Circular autoactivation loop antigen modified to form stable structure worked as a preventive metastasis vaccine in C57BL/6 mouse. (a) The amino acid sequence 604:627 of human Prss14/ST14 that contains an autoactivation cleavage site is well conserved. (b) The modeling of the aa604:627 structures from human and mouse Prss14/ST14 whole protein models (left two panels). aa604:627 peptide does not retain the original loop structure (Linear). A cysteine added to the 627th amino acid to form cysteine bond makes the loop structure (Loop). The activation cleavage site is indicated with arrows. (c) Preventive metavaccine effects of various peptide antigen forms. 1 × 106 E0771 cells were injected via tail vein of C57BL/6 mice after immunization of EP-SP, Mat-Loop, and Mat-Linear with Alum. (metastatic area/ whole area *100). (d) Metastasis inhibition of SP, Loop peptides, and KLH carrier protein. E0771 cells (1 × 106) were injected via tail vein after immunization with MF59. The lung weight was measured on the day of sacrifice. (E) The representative images of the metastasis. (f) The examination of antibodies (Left) and isotypes (Right)

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