Fig. 7

huAb3F3 clones inhibit cell migration and specifically bind to uncleaved protein. (a) Transwell migration assay with MCF7. 5 × 10⁴ cells were seeded on the upper chamber and incubated with antibody without serum. After 4 h, the lower chamber was filled with media with serum for 24 h. mAb5: Solid black line, m3F3 (mAb3F3): Solid red line, 3F3–35 (huAb3F3–35): Dotted orange line, 3F3–37 (huAb3F3–37): Dotted green line. (b) Flow cytometric analysis of HEK293TF cells co-transfected with either EGFP and matriptaseS805A or with tdTomato using huAb3F3 Ab clones. Blue population: tdTomato⁺, Red population: GFP⁺