Fig. 3
From: P53-R273H mutation enhances colorectal cancer stemness through regulating specific lncRNAs
Global profiling and identification of p53-R273H-regulated lncRNAs. a. Schematic illustration of the experimental approach and the bioinformatics analysis workflow of this study. b. Gene expression profiles of HCT116 p53-R273H PM spheroid cells or p53-ctrl spheroid cells. Genes (fold change ≥2 or ≤ 0.5, P < 0.05) are shaded in blue or orange in the heat map to indicate p53-R273H groups or p53-ctrl groups, respectively. c. Functional annotation clustering of 41 differentially expressed lncRNAs according to GO analysis. d. Signaling pathway based on KEGG enrichment analysis of 41 differentially expressed lncRNAs. e. The relative expression level of the top 10 lncRNAs in plastic state (PS) cells and spheroid state (SS) cells determined by RT-qPCR. Data are shown as means ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001. f. A heatmap of top 10 lncRNAs and CSC related genes. Red and blue represent correlation value greater than 0.95 or smaller than 0.8, respectively. R statistical software was used for visualization of the results