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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: circPTN sponges miR-145-5p/miR-330-5p to promote proliferation and stemness in glioma

Fig. 5

miR-145-5p and miR-330-5p are downregulated in glioma, inhibit glioma cells proliferation, and the inhibition of proliferation can be rescued by circPTN. a. miR-145-5p and miR-330-5p were significantly downregulated in glioma cell lines compared with HEB cells, n = 3, *p < 0.05, ANOVA. b. Data from GEO datasets (GSE4290) and Betastasis indicated that miR-145 and miR-330 were significantly downregulated in glioma tissues compared with NBT, respectively, *p < 0.05, t test. c. Stable overexpression system of miR-145-5p and miR-330-5p by lentiviral transfections in U87 and U251 cells, n = 3, *p < 0.05, t test. d. Results of CCK-8 assays suggested that miR-145-5p and miR-330-5p significantly inhibited proliferation of U87 and U251 cells, and enforcing expression of circPTN could rescue the inhibition of proliferation by miR-145-5p and miR-330-5p, n = 3, *p < 0.05, t test. e. Upper: Results of EdU assays showed that miR-145-5p and miR-330-5p significantly decreased the percentage of EdU-positive U87 and U251 cells, and enforcing expression of circPTN could rescue the decrease; scale bar, 100 μm. Lower: Analyses of EdU assays, n = 5, *p < 0.05, t test. f. Left: Cell cycle showed that miR-145-5p and miR-330-5p significantly arrest the shift of G1 → S, enforcing expression of circPTN could rescue the arrest in U87 and U251 cells; Right: Analyses of cell cycle results, n = 3, *p < 0.05, t test. g. The inhibition of SOX9/ITGA5 expression by miR-145-5p/miR-330-5p could be rescued by enforcing circPTN expression in U87 and U251 cells

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