Fig. 6
From: circPTN sponges miR-145-5p/miR-330-5p to promote proliferation and stemness in glioma
The expression of stemness markers in G15 cells and S15 cells. a. Left: G15 cells in suspension cultivation; Middle: G15 cells was cultivated on laminin and presented morphologically as short spindles; Right: S15 cells in adherent cultivation exhibited longer spindle morphology; scale bar, 100 μm. b. qPCR results of stemness markers in G15 cells and S15 cells, suggested that stemness markers significantly upregulated in G15 cells compared with that in S15 cells, n = 3, *p < 0.05, t test. c. Results of western blot assay showed stemness markers were higher in G15 cells than in S15 cells. d. Images of immunofluorescence for stemness markers in G15 cells and S15 cells. e. The expression of circPTN was significantly higher in G15 cells that in S15 cells, n = 3, *p < 0.05, t test. f. Cy3-labeled probe for circPTN in G15 cells and S15 cells by performing FISH. Images showed that circPTN majorly localized in the cytoplasm in G15 cells and S15 cells; scale bar, 10 μm