Fig. 6
PXN-AS1 acts as a sponge for miR-3064 to repress its expression in PC cells. (a) Putative miR-3064 binding site in PXN-AS1 sequence. (b) The expression level of PXN-AS1 in PC tissues and normal tissues from the TCGA PC datasets (the lncRNAtor database). (c) The expression of PXN-AS1 was measured in PC cell lines and a normal pancreatic cell line HPDE6-C7 using qRT-PCR analysis. (d) Luciferase reporter assays were conducted to confirm the direct binding between miR-3064 and PXN-AS1. (e) qRT-PCR analysis of miR-3064 expression in PC cells following knockdown or overexpression of PXN-AS1. (f) RIP assays were performed in PC cells transfected with or without miR-3064 mimic. The expression of PXN-AS1 was examined using qRT-PCR analysis. (g) Analysis of PXN-AS1 expression in PC tissues and normal tissues using qRT-PCR assays. (h) Kaplan-Meier curves for overall survival of PC patients with low or high PXN-AS1 levels. (i) Kaplan-Meier curves for overall survival of PC patients with high or low levels of PXN-AS1 (the GEPIA database). (j) Kaplan-Meier curves for overall survival of PC patients with high or low levels of PXN-AS1 (the KMPlotter database). *P < 0.05