Fig. 3

The regulatory role of normoxic and hypoxic exosomes in the proliferation, cell cycle distribution, migration and invasion of HUVECs. HUVECs were cultured with exosomes (25 μg /mL) from ECA109 that cultured in normoxic environment (norm-Exo (ECA109)) or hypoxic environment (hypo-Exo (ECA109)), or exosomes (25 μg /mL) from KYSE410 that cultured in normoxic environment (norm-Exo (KYSE410)) or hypoxic environment (hypo-Exo (KYSE410)), or in the absence of exosomes (Exosome (−)). The proliferation of HUVECs was detected by colony formation assay (a). The graph summarizes the results of three independent experiments (b). The cell cycle of HUVECs were analyzed by flow cytometry. Representative pictures of the cell cycle distributions in HUVECs (c). The graph summarizes the results of three independent experiments (d). Transwell assays were used to investigate the migratory (e) and invasive (g) abilities of HUVECs. The graph summarizes the results of three independent experiments of migration (f) and invasion assay (h). Data was presented as mean ± standard deviation (SD).*P < 0.05, **P < 0.01, ***P < 0.001