Fig. 2

Evaluation of the effect of RhoC on radiation response in cervical cancer cells. a Immunoblot analysis for expression of RhoC in cellular extracts of irradiated and non-irradiated SiHa cells showed that the irradiated cells have increased RhoC expression (n = 3). b Increased colony formation by 2.1-fold is shown in irradiated SiHa-R cells as compared to cells containing the control empty vector *p < 0.05. (n = 3). c-i Graphical representation of fold change in apoptotic cells upon RhoC knockdown followed by irradiation as analyzed by flow cytometry using Annexin V. Scr-Scrambled siRNA and RhoC -RhoC siRNA. (n = 3, *p < 0.005). c-ii Immunoblot analysis shows reduction in RhoC expression levels upon siRNA- based inhibition (n = 3). c-iii SiHa cells were irradiated 72 h post transfection with RhoC and scrambled siRNA and assessed for pH2Ax foci. Immunofluorescence analysis shows a decreased pH2Ax foci formation in cells with RhoC siRNA knockdown (scale bar = 10 μm). d(i-ii) Immunofluorescence analysis of SiHa-N and SiHa-R xenograft sections showed an increased expression of RAD50 and MRE11 proteins (scale bar = 50 μm)