Fig. 4

Transcriptional analysis highlights enrichment of DNA repair and survival pathway genes in ROCK2_hi cells. a A graphical representation of survival following irradiation in ROCK2_hi cells and ROCK2_lo cells. The viability was determined using the WST1 assay reagent. ROCK2_hi cells showed better survival. Fold ratio survival represented is normalized to that of the ROCK2_lo cells (n = 4, 1.4-fold, p < 0.03). b A graphical representation of increased migration of ROCK2_hi cells as compared to ROCK2_lo cells sorted based on ROCK2. *p < 0.03 (n = 3). c GO enrichment analysis of the selected biological processes in the ROCK2_hi cells with a p < 0.05 represented as a graph with -Log₁₀ P-values plotted on x-axis. d-i String interactome analysis was performed on the set of 51 genes that broadly represented DNA repair, cell cycle apoptosis and cell division. The network of DNA repair proteins formed a tight cluster represented in red colored nodes. The confidence level was set to 0.4 (medium). PPI enrichment p-value:< 1.0e-16. d-ii Heatmap of the representative genes that have been used in String analysis confirmed to be upregulated in the ROCK2_hi cells. Clustvis webtool based on R-software was used for the analysis. Color key shows the differential expression of the genes