Fig. 6

ROCK2_hi cells have an enhanced cell survival and are primed for a G2-M transition. a-i Representative histograms of flow cytometry analysis displaying the cell cycle profile of irradiated SiHa cells. The cell cycle plots were obtained after gating based on ROCK2 expression i.e., ROCK2 high expressing (ROCK2_hi) cells and ROCK2 low expressing (ROCK2_lo) cells. a-ii Graphical representation of distribution of cells in various phases of cell cycle, showing that the ROCK2_hi cells have a significant percentage of cells in G2/M phase as compared to the ROCK2_lo cells (n = 3). a-iii Graphical representation of percentage survival of ROCK2_hi cells and ROCK2_lo cells. ROCK2_hi cells shows a better survival (n = 3, * p < 0.02). b-i Graphical representation of the increased percentages of pAKT-Ser473 and P53-Ser15 in the SiHa cells following radiation treatment as compared to the untreated controls, *p < 0.03; **p < 0.03 (n = 3). b-ii Graphical representation of the distribution of pAKT-Ser473 and P53-Ser15 in ROCK2_hi population as compared to ROCK2_lo cells, gated based on ROCK2 expression levels in the irradiated SiHa cells, *p < 0.009; **p < 0.03 (n = 3). c-i Immunofluorescence staining showed an upregulated expression of both ROCK2 and CDK1 upon irradiation (scale bar = 10 μm). c-ii Flow cytometric analysis of irradiated SiHa cells showed an increased percentage of cells that co-expressed ROCK2 and CDK1 as compared to the corresponding control. c-iii Graphical representation of the distribution of pCDK1 levels in the ROCK2_hi population as compared to ROCK2_lo, gated based on ROCK2 expression levels in the irradiated SiHa cells, *p < 0.003 (n = 3)