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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: RhoC regulates radioresistance via crosstalk of ROCK2 with the DNA repair machinery in cervical cancer

Fig. 7

ROCK2 is the downstream effector of RhoC in radiation response. a-i An siRNA-based inhibition of RhoC in SiHa cells followed by immunoblotting of the cell extracts showed a reduction in the protein levels of RhoC and ROCK2 (n = 3). a-ii Immunoblotting the cell extracts of CaSki cells expressing the dominant negative RhoC (CaSki-dnR) showed a reduction in the ROCK2 levels as compared to the cells containing the empty vector (CaSki-N) (n = 3). b-i Immunoblotting of the cell extracts from SiHa cells with overexpression of RhoC showed an increase in the levels of ROCK2 as compared to the control cells (n = 3). b-ii Real time PCR showed a 2.4- fold upregulation of ROCK2 mRNA levels in SiHa-R cells as compared to SiHa-N cells (n = 3, p < 0.05). b-iii Graphical representation of flow cytometric analysis showed a 1.65-fold increase in the percentage of ROCK2 positive cells in the SiHa-R as compared to SiHa-N cells p < 0.05 (n = 3). c Graphical representation of flow cytometry analysis of SiHa-R cells with ROCK2In displayed loss of survival advantage post-irradiation as compared to IgGIn cells (n = 3, *p < 0.03). d Immunoprecipitation using RhoC antibody resulted in pulldown of ROCK2 (~ 120 kDa). e Representative images of immunofluorescence-based analysis of xenografts derived from SiHa-N and SiHa-R tumors showing increased expression of ROCK2 in SiHa-R derived sections (scale bar = 50 μm)

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