Fig. 4
CR-1 promotes ccRCC cell proliferation and tumorigenicity in vitro and in vivo. a CR-1 protein is increased after over-expression of CR-1 in Caki-2 cells and decreased after knockdown of CR-1 in 786-O and Caki-1 cells. b and c Ectopic expression of CR-1 stimulates cell proliferation in Caki-2 cells whereas knockdown of CR-1 inhibits cell proliferation in 786-O and Caki-1 cells as determined by MTT assays (b) and colony formation assays (c). Data represent the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 by two-sided unpaired Student’s t-test. d Cell cycle analysis was performed by flow cytometry for indicated cell lines. The percentage of cell population at different cell cycle phases are shown in the histograms. **P < 0.01 by two-sided unpaired Student’s t-test. e Effects of CR-1 knockdown on tumor growth in vivo. Caki-1/LV-shNC or Caki-1/LV-shCR-1 cells (5 × 106) were injected subcutaneously into nude mice (5 mice /group). Left panel, tumor growth curves. Right panel, images of tumor xenografts. Data are represented as mean ± SD. **P < 0.01 by two-sided unpaired Student’s t-test. f Comparison of weight of harvested xenografts. Data are presented as means ± SD. **P < 0.01 by two-sided unpaired Student’s t-test. g Histopathology of xenograft tumors, the tumor sections were under IHC staining for CR-1 and Ki-67