Fig. 6

CR-1 promotes EMT in ccRCC cells by activating the Wnt/β-catenin signaling in vitro and in vivo. a After overexpressing CR-1 in Caki-2 cells or downregulating CR-1 expression in 786-O and Caki-2 cells, the protein levels of E-cadherin, N-cadherin, Vimentin, ZEB-1, Snail, MMP-2, and MMP-9 were measured by Western blot. b IF was used to compare the expression levels of E-cadherin, N-cadherin, and Vimentin between Caki-2/LV- vector and Caki-2/LV- CR-1, 786-O/LV-shNC and 786-O/LV-shCR-1, and Caki-1/LV- shNC and Caki-1/LV-shCR-1 cells. c CR-1 knockdown reduced the expression of p-GSK3β, β-catenin, C-myc, and cyclin D1; in contrast, CR-1 upregulation increased the expression of p-GSK3β, β-catenin, C-myc, and cyclin D1. d Expression of EMT markers (E-cadherin, N-cadherin and Vimentin,) and Wnt/β-catenin signaling genes (β-catenin, p-GSK3β, C-myc, and cyclin D1) was detected by Western blot using xenograft tumors from Caki-1/LV- shNC (n = 5) and Caki-1/LV-shCR-1 (n = 5) groups