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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Long noncoding RNA 00976 promotes pancreatic cancer progression through OTUD7B by sponging miR-137 involving EGFR/MAPK pathway

Fig. 2

Linc00976 promotes cell proliferation of PC cells in vitro. a qRT-PCR analysis showing Linc00967 expression after transfection of Linc00976-knockdown (Linc00976D), Linc00976-overexpression (Linc00976U) and negative control (NC) in PANC-1 and MIA PaCa-2 cells infected by lentiviral infection. b CCK-8 assay comparing cell proliferation of Linc00976D, Linc00976U and NC group in PANC-1 and MIA PaCa-2 cells. c Colony formation experiment analysis the cell colony growth of Linc00976D, Linc00976U and NC group in PANC-1 and MIA PaCa-2 cells. d EDU assay analysis the proliferation capacity of Linc00976D, Linc00976U and NC group in PANC-1 and MIA PaCa-2 cells. e Effects of Linc00976 overexpression and Linc00976 knockdown on cell cycle progression in PANC-1 and MIA PaCa-2 cells. f Western-blot analysis the cell cycle associated protein Cyclin D1 and CDK2 expression of Linc00976D, Linc00976U and NC group in PANC-1 and MIA PaCa-2 cells. The data are shown as means ± S.D. of three independent assays (*P < 0.05, ** P < 0.01, *** P < 0.001)

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