Fig. 7

Linc00976-miR-137/OTUD7B axis on the EGFR downstream MAPK pathway. a and b The predicted potential binding site of miR-137 with wild-type Linc00976 (Linc00976-WT) and OTUD7B(OTUD7B-WT), and the designation of mutant of Linc00976 (Linc00976-MUT) and OTUD7B(OTUD7B-MUT) according to the seed region of the miR-137 binding site. c and d The Spearman rank correlation showed that negatively correlation with miR-137and Linc00976, OTUD7B. e and f Luciferase reporter assay analysis that the interaction with miR-137 and Linc00976, OTUD7B. g qRT-PCR showed that miR-137 overexpress and OTUD7B downregulated could partly restrained the mRNA express level of MAPK target genes (CyclinD1, CDK2, MMP2, MMP9) in Linc00976U PC cells. h Western-blot assay analysis of Linc00976/miR-137/OTUD7B axis on the EGFR downstream pathway MAPK activity