Table 1 The characteristics of the primers used for real-time PCR and plasmid sequence
Gene | Sequence (5′ - > 3′) | |
|---|---|---|
Linc00976 | Forward Primer | CAGCAGTGTGAAGCAATG |
Reverse Primer | ACAAGTAGAGTCTATGGTTCC | |
OTUD7B | Forward Primer | GTCAGATTTTGTCCGTTCCACA |
Reverse Primer | CATGGACTTGACGTAGCTGTT | |
CyclinD1 | Forward Primer | GCTGCGAAGTGGAAACCATC |
Reverse Primer | CCTCCTTCTGCACACATTTGAA | |
CDK2 | Forward Primer | CCAGGAGTTACTTCTATGCCTGA |
Reverse Primer | TTCATCCAGGGGAGGTACAAC | |
MMP2 | Forward Primer | TACAGGATCATTGGCTACACACC |
Reverse Primer | GGTCACATCGCTCCAGACT | |
MMP9 | Forward Primer | TGTACCGCTATGGTTACACTCG |
Reverse Primer | GGCAGGGACAGTTGCTTCT | |
Control | UUAUUGCUUAAGAAUACGCGUAG | Â |
UCACAACCUCCUAGAAAGAGUAGA | Â | |
miR-137 inhibitor | CTACGCGTATTCTTAAGCAATAA | Â |
miR-137 mimic | UUAUUGCUUAAGAAUACGCGUAG | Â |
Si-Linc00976 | 5′-GTCAATTGCTCAAACCAAA-3’ |  |
Si-OTUD7B | GCAAAGAUGAUAGUGACAATT | Â |
| Â | UUGUCACUAUCAUCUUUGCTT | Â |