Fig. 2

LINC00174 regulated cell proliferation and apoptosis in vitro and in vivo. a U251 and LN229 cells were transfected with pcDNA3.1 or pcDNA3.1-LINC00174, and LINC00174 expression was examined by RT-qPCR. b U251 and LN229 cells were transfected with pLKO.1, or pLKO.1-LINC00174#1, or pLKO.1-LINC00174#2, and LINC00174 expression was examined by RT-qPCR. c Cell proliferation was examined by CCK8 assay. d Cell apoptosis was identified by TUNEL analysis. e The effect of LINC00174 on tumor growth was examined by a nude-mouse transplanted tumor model. Tumor growth curves were established by measuring tumor volume every 3 for 21 days after injection. Tumor weights isolated from nude mice in each treatment group were determined on day 21 after injection. f Ki67 expression in tumor tissues were asses by IHC analysis. Data are presented as the mean ± SD. **P < 0.01, and ***P < 0.001