Skip to main content
Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Long non-coding RNA LINC00174 promotes glycolysis and tumor progression by regulating miR-152-3p/SLC2A1 axis in glioma

Fig. 5

The miR-152-3p targeted SLC2A1 to regulated cell physiological activities of glioma cells. a miR-152-3p binding sites in SLC2A1 predicted by bioinformatics analysis. b Dual luciferase reporter assay was performed to identify SLC2A1 directly combined with miR-152-3p. c Glioma cells were transfected with miR-152-3p mimics or inhibitors. d-e The mRNA and protein expression of SLC2A1 expression in glioma cells with miR-152-3p mimics or inhibitors transfection was examined by RT-qPCR and western blot. f The SLC2A1 expression in glioma tissues was detected, and showed a negative correlation with miR-152-3p expression. g U251 cells were transfected with NC mimics, or miR-152-3p mimics, or miR-152-3p mimics + pcDNA3.1, or miR-152-3p mimics + pcDNA3.1-SLC2A1, SLC2A1 expression was examined by western blot analysis. h-l Cell proliferation, apoptosis, migration, invasion and glycolysis were then identified by CCK8, TUNEL, wound healing, transwell and ELISA, respectively. Data are presented as the mean ± SD. **P < 0.01, ***P < 0.001

Back to article page