Fig. 4

ACLY affects epithelial-mesenchymal transition (EMT) of CRC in vitro. a, b Image showed morphological changes of HCT116 and RKO cells stably knockdown ACLY vs control cells (magnification, 200×). c-f Quantitative real-time PCR (qPCR) analysis to qualify the endogenous mRNA levels of EMT-associated markers (c, e) and transcriptional factors (d, f) in HCT116 and RKO cells ACLY deficiency or the control. g, h Western blot to determine the protein levels of EMT markers and transcriptional factors in HCT116 and RKO cells ACLY deficiency or the control. Actin was the loading control. i Immunofluorescence assays showed the E-Cadherin protein level in HCT116 control cells was lower than KO cells (magnification, 200×)