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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: A novel BMI-1 inhibitor QW24 for the treatment of stem-like colorectal cancer

Fig. 2

QW24 decreased the protein level of BMI-1 by reducing protein stability. a and b, HCT116 cells were treated with indicated concentrations of QW24 for 12 h (a) or 2 μM QW24 for indicated time (b). Cells were lysed and BMI-1 protein level was measured by western blotting analysis. c-f. HCT116 cells were treated with indicated concentrations of QW24 for 12 h (c, d) or 2 μM QW24 for indicated time (e, f). Then BMI-1 mRNA level was measured by quantitative-PCR. The agarose gel (c, e) and quantitative-PCR (d, f) were run to quantify the mRNA level of BMI-1. Data are presented as mean ± s.d. (n = 3) in quantitative-PCR; n.s., Not statistically significant. g, HCT116 cells were treated with 50 μg/ml protein synthesis inhibitor cycloheximide (CHX) with or without 2 μM QW24 (or 2 μM PTC-209) for indicated lengths of time. BMI-1 protein level was measured by western blotting analysis

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