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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: A novel BMI-1 inhibitor QW24 for the treatment of stem-like colorectal cancer

Fig. 5

QW24 suppressed the colorectal tumor growth in subcutaneous xenograft model and down-regulated BMI-1 in vivo. a-d, HCT116 cells were injected into the right flank of BALB/c-nude mice. When the volumes of tumor nodules reached 100 mm3, the mice were randomly assigned to indicated groups and respectively i.p. injected with DMSO (n = 10), PTC-209 30 mg/kg (n = 10), QW24 15 mg/kg (n = 10) and 30 mg/kg (n = 10). After 24 days treatment, mice were sacrificed and tumors were harvested. Tumors image was photographed (a) and tumors were weighed (c). Tumor volume (b) and the mice body weight (d) were measured every 4 days. Scale bar, 1 cm. Data are presented as mean ± s.d. (n = 10); **, P < 0.01; ***, P < 0.001. e, Three representative tumor samples per group as indicated were lysed. BMI-1 protein level was measured by western blotting analysis. f, Images of xenografts treated with DMSO (control) and 30 mg/kg QW24 with corresponding IHC for Ki67, PCNA and BMI-1 were shown. The cropped region from each image is to enable a closer view. Scale bars, 100 μm or 50 μm as indicated

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