Fig. 1

FAM289 was overexpressed in GBM cell lines and tissues. (A) FAM92A1 expression levels were increased in human brain cancer cells and tissues by analyzing the GBM RNA sequencing dataset from TCGA. (a) FAM92A1 expression levels were increased in human brain cancer cells compared to other kinds cancer cell lines (1. Bladder Cancer (n = 21) 2. Brain and CNS Cancer (n = 64) 3. Breast Cancer (n = 56) 4. Colorectal Cancer (n = 56) 5. Esophageal Cancer (n = 25) 6. Gastric Cancer (n = 35) 7. Head and Neck Cancer (n = 41) 8. Kidney Cancer (n = 21) 9. Leukemia (n = 83) 10. Liver Cancer (n = 29) 11. Lung Cancer (n = 166) 12. Lymphoma (n = 61) 13. Melanoma (n = 57) 14. Myeloma (n = 26) 15. Ovarian Cancer (n = 44) 16. Pancreatic Cancer (n = 44) 17. Prostate Cancer (n = 7) 18. Sarcoma (n = 39)). (b) Geometric mean of the FAM92A1 expression was significantly higher for GBM tissues compared with normal brain samples in three independent datasets. (c) Kaplan–Meier analysis of overall survival curves of FAM2A1 expression and clinical outcomes from the TCGA database. (B) The mRNA levels of FAM289 in different degrees of malignancy GBM cells lines (U251, DBTRG, U87-MG) was analyzed by western blotting (a) and qRT-PCR (b). (C) Overexpression of FAM289-GFP fusion protein in U251 cells was verified by fluorescence microscopy (a) and western blotting (b). (D) FAM289 was knocked down in U87-MG cell lines by siRNA transfection (a-c) or LentiCRISPRv2 –FAM289 infection (d-f). NABT: normal brain tissue; KD: knock down; siRNA: small interfering RNA; sgRNA: small guide RNA; Con: control; OE: overexpression; M: 100 bp DNA ladder