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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Long non-coding RNA HOTTIP enhances IL-6 expression to potentiate immune escape of ovarian cancer cells by upregulating the expression of PD-L1 in neutrophils

Fig. 5

HOTTIP enhances PD-L1 expression in neutrophils, thereby contributing to immune escape of OC cells. The mice (5 in each group) were intraperitoneally injected respectively with 100 μL PBS, neutrophils + T cells (5 × 106 cells for either;, 1: 1, a total of 100 μL), SKOV3-neutrophils + T cells (5 × 106 cells for either; 1: 1, a total of 100 μL), SKOV3-neutrophils overexpressing HOTTIP + T cells (5 × 106 cells for either; 1: 1, a total of 100 μL), or PD-L1 antibody (20 μg/mL)-treated SKOV3-neutrophils overexpressing HOTTIP and T cells (5 × 106 cells for either; 1: 1, a total of 100 μL). a The body weight of mice after treatment. b The tumor volume of mice after different treatment. c Immunohistochemical staining of CD3+ T cell infiltration (400 ×). d The expression of IFN-γ+ in CD3 T+ cells as detected by flow cytometry. * p < 0.05 vs. the SKOV3-neutrophils + T cells. The measurement data were summarized as mean ± standard deviation. The one-way analysis of variance was adopted to compare the data among multiple groups, followed by Tukey’s post hoc test. Repeated measures analysis of variance was applied to compare the data at different time points, followed by Bonferroni post hoc test. n = 5. The experiment was repeated 3 times independently

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