Fig. 4

circDENND4C is a sponge of miR-200b/c. a and b The potential binding sites of circDENND4C and miR-200b/c were predicted by starBase. c and d Luciferase activity was analyzed in MDA-MB-453 and SK-BR-3 cells co-transfected with miR-200b mimic (miR-200b), miR-200c mimic (miR-200c) or miRNA negative control (miR-NC) and wild-type (WT) or mutant (MUT) circDENND4C luciferase reporter vector (circ-WT or circ-MUT). e and f The level of circDENND4C (circ) enriched by RIP was detected in MDA-MB-453 and SK-BR-3 cells transfected with miR-200b, miR-200c or miR-NC. **P < 0.01, ***P < 0.001