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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Methylation-mediated silencing of miR-133a-3p promotes breast cancer cell migration and stemness via miR-133a-3p/MAML1/DNMT3A positive feedback loop

Fig. 5

miR-133a-3p promotes breast cancer cells migration and invasion via targeting MAML1. a, b Migration of MCF-7 cells and MDA-MB-231 cells transfected with mimics NC plus pcDNA3.1, miR-133a-3p mimics plus pcDNA3.1, or miR-133a-3p mimics plus pcDNA3.1-MAML1 (a), and inhibitor NC plus si-NC, miR-133a-3p inhibitor plus si-NC, or miR-133a-3p-inhibitor plus si-MAML1–2 (b) detected by wound healing assay. Scale bar, 100 μm. c, d Migration and invasion of MCF-7 cells and MDA-MB-231 cells transfected with mimics NC plus pcDNA3.1, miR-133a-3p mimics plus pcDNA3.1, or miR-133a-3p mimics plus pcDNA3.1-MAML1 (c), and inhibitor NC plus si-NC, miR-133a-3p inhibitor plus si-NC, or miR-133a-3p-inhibitor plus si-MAML1–2 (d) detected by transwell migration and invasion assay. Scale bar, 100 μm. e, f The protein levels of MAML1, Notch signaling and EMT target genes in MCF-7 cells (left) and MDA-MB-231 cells (right) transfected with mimics NC plus pcDNA3.1, miR-133a-3p mimics plus pcDNA3.1, or miR-133a-3p mimics plus pcDNA3.1-MAML1 (e), and inhibitor NC plus si-NC, miR-133a-3p inhibitor plus si-NC, or miR-133a-3p-inhibitor plus si-MAML1–2 (f). g, h Proliferation of MCF-7 cells (g) and MDA-MB-231 cells (h) transfected with either the mimics NC plus pcDNA3.1, miR-133a-3p mimics plus pcDNA3.1, or miR-133a-3p mimics plus pcDNA3.1-MAML1 detected by CCK8 assay. *P < 0.05; **P < 0.01; ***P < 0.001

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