Fig. 2

TRIP13 promoted cell proliferation, migration and invasion of HCC cells in vitro. a Western blot analysis of TRIP13 in six different HCC cell lines. b The efficiency of transfection in SMMC7721 and MHCC97H cell lines were confirmed by western blot. c-d Knockdown of TRIP13 in MHCC97H cells inhibited cell proliferation as determined by CCK-8 (c) and colony formation assays (d). e-f Overexpression of TRIP13 in SMMC7721 cells promotes cell proliferation as determined by CCK-8 (e) and colony formation assays (f). g-h Interference with TRIP13 expression in MHCC97H cells inhibited cell migration and invasion as determined by wound healing assay (g) and transwell assay (h). i-j Overexpression of TRIP13 in SMMC7721 cells promotes cell migration and invasion as determined by wound healing assay (i) and transwell assay (j). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001