Fig. 1

TP53-mutated melanoma show higher CD274 mRNA expression levels, and are immunohistochemically more often PD-L1 positive. a Analysis of TCGA melanoma samples (n = 347) for differences in CD274 mRNA expression. Wilcoxon-Mann-Whitney test was used to compare CD274 expression between TP53-wt and -mutant samples. b PD-L1 immunohistochemistry of primary and metastatic melanoma samples (n = 81). Next-generation sequencing of the TP53 gene was performed previously. Samples harboring more than > 1% PD-L1 positive melanoma cells were regarded as PD-L1-positive. A PD-L1 negative, and a positive metastasis are depicted. Magnification, each 400x (c, d) Samples (n = 262) were stratified by TP53-mutation status and publicly available PD-L1 expression determined by reverse phase protein array was compared with the Wilcoxon-Mann-Whitney test (c) and by linear regression association analysis with p53 (d). mRNA and protein data are presented on a logarithmic scale. p < 0.05 is regarded as statistically significant. TPM, transcripts per million; wt, wildtype; mut, mutation