Fig. 2

CD274 expression correlates with genes of immune-related pathways while PD-L1 levels lack correlation with p53’s transcriptional activity. a Immunoblot for PD-L1 and p53 expression of each six untreated TP53-wildtype and -mutated melanoma cell lines. Before lysis, cells were cultured for at least three days. Blot is representative of two individual experiments. b Correlation of CD274 with other genes was calculated using Spearman correlation (n = 447). Only genes with > 1 TPM in a least 20% of cases were included. The 24 best correlating genes are presented in a “heatmap”. Expression values are presented in a spectrum of blue (small) to red (high). c Gene enrichment analysis of the 24 best correlating genes with PANTHER overrepresentation test and Fisher test statistics depicting overrepresented pathways. Total number (#) of genes ascribed to the respective pathway, number of genes from the top 24 genes belonging to the pathway, expected frequency, fold enrichment, overpresentation indicated by “+”, raw p-value and false discovery rate (FDR) are given. p < 0.05 is regarded as statistically significant. TPM, transcripts per million