Fig. 4
From: IFN-gamma-induced PD-L1 expression in melanoma depends on p53 expression
Correlation of STAT1 and CD274/PD-L1 in melanoma, and disturbed IFN-ɣ signaling upon p53 knockdown in melanoma cell lines. a, b Linear regression analyses of STAT1 mRNA with CD274 mRNA (n = 347) (a) or PD-L1 protein (n = 262) (b). c LOX-IMVI and M19-MEL were incubated in six well plates for 6 days with doxycycline for p53 knockdown and subsequently treated with IFN-ɣ for 5, 15, 30 or 60 min. Effect of treatment on the IFN-ɣ signaling pathway was analyzed by immunoblot with the indicated antibodies. ß-tubulin served as a loading control. p < 0.05 is regarded as statistically significant. DOX, doxycycline; TPM, transcripts per million