Fig. 5

PCR array analysis of gene expression in cl-amidine combined with docetaxel treatment in MCF7/TamR cells in comparison to control cells. a Volcano plot human RT² Profiler PCR Cell for cell apoptosis array, cell cycle array, and autophagy array. The relative expression levels for each gene depicted as log 2 (n-fold) plotted against –Log10 (P-value). Arrows indicating significantly upregulated or downregulated genes. b qRT-PCR analysis validated the expression of the genes selected from the PCR arrays in (a). c Gene ontology (GO) function analysis using DAVID Bioinformatics Resources (http://david.abcc.ncifcrf.gov/) confirming that the genes regulated by the combination treatment were enriched in p53 signaling pathway in cancers (as indicated by arrow)